The phenotypic screening facility house all the equipment, software and support needed to facilitate a broad spectrum of screening project. Whether you would like to screen the whole genome or simply automate image acquisition, we have some of the most advance, fully automated systems available. The facility is open to all staff across QMUL and external users and is fully supported by dedicated staff.
The facility is in three main sections:
To date, the facility has successfully completed a number of high-content miRNA, genome-wide siRNA and compound screens (see Publications).
The imaging equipment within the facility also forms part of the AMIS umbrella which is a strategic investment to draw together state-of-the-art advanced molecular imaging equipment acorss QMUL. More information can be found here.
The Cybio is a liquid handling system that is full programmable allowing flexible assay development for screening projects.
As well as housing all the equipment required for your screen we also house a number of screening libraries ready to use.
FDA Approved oncology Drug Set VII –
The INCA 2200 is a super-fast full automated and sensitive wide-field imaging system.
The INCA6000 is a laser based automated confocal instrument that has a fully adjustable aperture to match your imaging needs
Manual nuclei counting or measuring the area covered by foci with programs like ImageJ can be incredibly tedious and time consuming. Fully definable high content analysis software removes this bottleneck allow for bespoke protocols to be written that will give you phenotypic data on a per object (foci, nuclei, cell), per field and per well bases. Once written the protocol can be set to run and report back the data with a simple excel file.
Data storage is often an afterthought when applying for grants and planning a screen. High throughput instruments can take an incredible number of high quality images very quickly. Combine this with 3D imaging, time series or large scale screen projects and you will generate several TBs of data. Within the screening facility we house our own data storage to accommodate even the largest of projects.
With the ability to measure real-time oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) the Seahorse allows metabolic screening of your cultures
Luke Gammon (Facility Manager)
Desk: 0207882 2342
Screening Room: 0207882 2404
Cleo Bishop (Academic Lead)
For training or booking please contact facility manager (Luke Gammon) or by using the iLabs Booking system https://qmul.corefacilities.org/service_center/show_external/3579
|Equipment||Internal (p/H)||External (p/H)||Internal (O/N 5pm-9am)||External (O/N 5pm-9am)|
|Analysis (Developer)||Free||Please contact core manager||Free||Please contact core manager|
|Analysis (INCarta)||Free||Please contact core manager||Free||Please contact core manager|
|Cybio Liquid Handling System||Please contact core manager||Please contact core manager||Please contact core manager||Please contact core manager|
|Screening Libraries||Please contact core manager||Please contact core manager||Please contact core manager||Please contact core manager|
|Seahorse||Free||Please contact core manager||Free||Please contact core manager|
Please contact us to discuss charges for extended imaging times such as large scale screening projects or long-term live cell imaging