Flow cytometry is a powerful tool for the multiparameter analysis of cells of all types. Three high powered high speed flow cytometers are available as well as one simpler cytometers and a three colour LED upright Fluorescence microscope in the Blizard Institute. The Blizard Institute instruments include a four laser 16 fluorochrome BDAria IIIu cell sorter and a five laser Cytek Aurora spectral analyser with 40 fluorochrome channels, five laser 18 fluorochrome analyser a BD LSRII, a three laser 8 fluorochrome BD FACSCanto II and a 3 laser 13 fluorochrome ACEA Biosciences Novocyte 3000.
Training and assistance will be provided by the Flow Cytometry Core Facility Manager.
NEW MIFlowCyte criteria for good practice in data publication, see Cytometry the scientific journal for the International Society for the Advancement of Cytometry, ISAC, see details and download form in Flow Cytometry Guides. All such data for publication in Cytometry has to be uploaded to FlowRepository.org
All cell samples for sorting must be assessed for pathogenic and GM potential. Thus it is also required to supply a BioCOSHH form assessment of the cells due for sorting. Fixed samples can be sorted after the fixative is removed. More information can be found on the link, biological hazard. Cell sorting assessment form http://www.icms.qmul.ac.uk/flowcytometry/contacts/biologicalhazard/FACS%20Cell%20Sorting%20Assessment.pdf), Cell Sorting Biosafety Guidance, and QMUL Biological Safety Procedures documentation.
A REMINDER to all staff that the Flow Cytometry Core Facility offers NEW ASSAY DEVELOPMENT expertise, click here for list of new assay's already developed. Flow cytometry may be the answer to a research project problem and we can investigate whether flow cytometry can be used to investigate your research demands.
Expert support and technical assistance is also available to help with the design and development of experiments. Please see contacts for help and advice. Academic Lead for this core facility is Pro Dan Pennington, lead for the Centre for Immunobiology.
NEW BOOKING SYSTEM FOR 2017 Blizard Institute use only:- Please pre-register and add grant code to your account and click here for on-line booking with iLabs https://qmul.corefacilities.org/signed_out
ICMS Staff and associated Institutes
£30 per hour for BDLSR II, BDCanto II and ACEA Biosciences Novocyte 3000 analysis
£10 per hour for the Millipore Muse
£50 per hour for assistance
£50 per hour for cell sorting
£50 per hour for analysis
£85 per hour for assistance
£100 per hour for sorting
£70 per hour for analysis
£120 per hour for assistance
£175 per hour for sorting
Dr Gary Warnes, Flow Cytometry Core Facility Manager Tel: 020 7882 2402 email: firstname.lastname@example.org
Prof Dan Pennington, Research Lead, Centre for Immunobiology Tel: 020 7882 2302 email: email@example.com
Blizard Users for on-line booking please go to iLabs https://qmul.corefacilities.org/signed_out