BD FACScan Analyser
| Specification | Comments |
| Performance | |
| Fluorescence sensitivity | <2,000 molecules of equivalent soluble fluoroscein |
| Fluorescence resolution | < 3% for PI labelled chick erythrocyte nuclei |
| Forward and side scatter sensitivity | Sensitivity enables the separation of fixed platelets from noise |
| Forward and side scatter resolution | 5,000 events/sec |
| Excitation optics | |
| Optical platform | Fixed optical assembly, |
| Lasers | 20mW Coherent 488nm, air cooled, argon ion laser FITC (530/30nm); PE (575/26nm), PerCP, or PE-Cy5,or PE-Cy5-5 or PE-Cy7 (650nmLP) |
| Emission optics | |
| Optical Coupling | Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency |
| Fluorescence Detectors | 3 PMTs |
| Three fluorescence detectors | High performance, high dynamic range photomultipliers with filters for FITC, PE and PE-Cy5 or PE-Cy7 |
| Signal processing | |
| Workstation resolution | Four decades for peak detection, |
| Data acquisition channels | Five acquisition channels |
| Dynamic range | 12-bit data acquisition 1,024linear channels displayed in a 4-decade logarithmic display |
| Pulse processing | Not available |
| Time | Time available correlated to any parameter |
| Analogue Compensation | Electronics provides analogue correction of spectral overlap |
| Data management | |
| Central processing | Intel processor |
| CellQuest Pro II |
Apple Mac G5 computer with OSX operating system |
| Monitor | 15-inch LCD monitors |
| Data File Structure | Flow Cytometry Standard 2.0 |