BD FACS Canto II
| Specification | Comments |
| Performance | |
| Fluorescence sensitivity | <200 molecules of equivalent soluble fluoroscein |
| Fluorescence resolution | < 3% for PI labelled chick erythrocyte nuclei |
| Forward and side scatter sensitivity | Sensitivity enables the separation of fixed platelets from noise |
| Forward and side scatter resolution | >10,000 events/sec |
| Excitation optics | |
| Optical platform | Fixed optical assembly, kinematic beam steering |
| Lasers | 20mW Coherent Sapphire 488nm, air cooled, argon ion laser: 530/30nm; 585/42nm; 670nmLP; 780/60nm. 25 mW 405 Coherent VioFlame; 450/50nm; 510/50nm 20 mW 635 JDS Uniphase HeNe; 660/20nm; 780/60nm. |
| Emission optics | |
| Optical Coupling | Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency |
| Fluorescence Detectors | Three fixed fibre apertures |
| Eight fluorescence detectors | High performance, high dynamic range photomultipliers with filters |
| Signal processing | |
| Workstation resolution | Five decades for peak detection, offset less than 1/10,000 full scale |
| Data acquisition channels | Ten acquisition channels with up to three time-displaced laser beams |
| Dynamic range | 18-bit data acquisition with IEEE 32 bit floating-point resolution 262,144 linear channels displayed in a 5-decade logarithmic display |
| Pulse processing | Width and area measurements available for all fluorescence parameters Ratio measurements for intra-laser parameters |
| Time | Time available correlated to any parameter |
| Channel triggering | Available on any laser signal |
| Data management | |
| Central processing | Dual-Core Intel Xeon processors |
| Data storage | 2GB RAM and 120-GB internal hard drive, internal drive with 250-MG removable disk storage space capacity, 3.5-inch floppy drive, CD-RW drive. |
| Monitor | 24-inch LCD monitor |
| Software | BD FACSDiva v6.1.3, BD FACSCanto clinical v6.1.03 |
| Data File Structure | Flow Cytometry Standard 3.0 or 2.0 |