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Blizard Institute - Faculty of Medicine and Dentistry

Gene Expression

Individual gene expression analysis

The Genome Centre provides analysis of individual transcripts using the ABI 7900HT real time PCR instrument. 

Individual genes or combinations of genes can be assayed using Taqman probes targeting specific exon/exon boundaries. Automatic set up using a Beckmann Biomek FX in 384 well plates in a 10µl volume ensures accurate, reproducible, cost effective results. We recommend that reference genes are selected empirically following the method of Vandesompele et al 2002. A selection of assays from LifeTechnologies for six common reference genes are available at the Centre for human, mouse and rat.

Larger numbers of genes, from 24 to 382 can be assayed simultaneously using MicroFluidic Cards in either off the shelf panels (TaqMan® Gene Signature Arrays) or in customisable format.

Samples can be provided either as total RNA (which will be quality controlled using a nanodrop and Agilent BioAnalyser on receipt) or as cDNA.

Reference genes are available as Taqman probes for LifeTech. See the table below for details:

18s RNA Hs99999901_s1 Hs99999901_s1 Hs99999901_s1
ATP Hs00969567_g1 Mm00443967_g1 Rn01756316_g1
B2M Hs99999907_m1 Mm00437762_m1 Rn00560865_m1
EIF4A2 Hs00756996_g1 Mm00834357_g1 Rn01404755_g
GAPDH Hs99999905_m1 Mm99999915_g1 Rn99999916_s1
UBC Hs00824723_m1 Mm01201237_m1 rRn01789812_g1
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