Extraction
DNA extraction and purification methods used at the Genome Centre
The Genome Centre offers DNA extraction from most common sources including blood, saliva and solid tissue. We have worked on a number of these with our robotics supplier, Beckman Coulter, to automate these, increasing accuracy and efficiency. The table below shows some of the options for DNA extraction. For more information and to discuss options please contact Dr Charles Mein on 020 7882 2055.
| Source | Extract | Format | Method |
|---|---|---|---|
| Whole blood | DNA | Single tubes to 10ml or 96 samples at 800µl | Salting out1 protocol, manual or automated on a Biomek FX |
| Whole Blood | DNA | 96 well format 200µl | Wizard SV 96 Genomic DNA purification system, automated on the Biomek FX |
| Saliva | DNA | Single Sample or batches of 96 samples | Oragene™ technology manual protocol for small numbers of samples or fully automated for batches of 96 |
| Solid Tissue | DNA | 96 well format | Wizard SV 96 Genomic DNA purification system, automated on the Biomek FX |
| Plasmid DNA preparation | DNA | 96 well format | Wizard SV 96 Genomic DNA purification system, automated on the Biomek FX |
| Solid Tissue | RNA | Single Tubes | Qiagen RNeasy manual protocol |
| Cultured Cells | RNA | Single Tubes | Qiagen RNeasy manual protocol |
1Miller et al 1988, Nucleic acids Research 16, 1215
In addition, we can work with collaborators to develop novel techniques for extraction from other sources of DNA and RNA.