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Research

Zeiss LSM 880 Laser Scanning Confocal Microscope (inverted) with Fast Airyscan module


State-of-the art, fast scanning, near super-resolution confocal microscope. The Airyscan detector makes it possible to increase the resolution of your imaging far beyond the resolution of a classic confocal point scanning microscope. In addition, images can be acquired with high sensitivity and high speed.

This core confocal microscope facility was made possible by a grant from Barts Charity

Fast scanning: 27 frames/sec @ 480 x 480 pixels.
Resolution: max 140nm lateral and 400nm axial resolution.
Light source: The system is equipped with a Diode 405nm laser, a Multiline Argon laser (wavelengths 458nm, 488nm and 514nm), a DPSS 561nm laser, a HeNe 594nm laser and a HeNe 633nm laser. Additionally, a transmitted light detector is available.
Detectors: The confocal has a range of ultra-sensitive detectors (2x PMT, 1x GaAsP, 1x Airyscan).

Available objectives:

  • Plan-Apochromat, 10x (NA: 0.45; WD:2.0), Dry
  • Plan-Apochromat, 20x (NA: 0.80; WD:0.55), Dry
  • Plan-Apochromat, 40x (NA: 1.3; DIC UVVIS-IR), Oil
  • Plan-Apochromat, 63x (NA: 1.4; DIC f/ELYRA), Oil

Software:

  • ZEN Black software interface
  • Modules for 3D/4D reconstruction and animation; spectral scanning; analysis and quantification of co-localisation; spectral unmixing, advanced tiling and photo-bleaching (FRAP, FRET).

Other features:

  • An incubation system with temperature and CO2 control.
  • Fully motorised stage
  • Piezo z-drive for accurate high-speed z-stacks

Costs

Price per hour : £25

Price O/N :

Contact details

Facility : Endocrinology

Campus : WHRI (1st floor) CHS

Address : Endocrinology, John Vane Building, Charterhouse Square, London, EC1M 6BQ

Manager : Lisa Romano & Paul Chapple

Contact : 020 7882 6242

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