Leica TCS SP5 laser scanning confocal microscope with two photon capability

Leica TCS SP5 confocal microscope system is equipped with a number of lasers for probe excitation between 355 nm and 633 nm.A steplessly tunable detector system can record any wavelength emitted from the sample and up to five channels may be recorded simultaneously.Two different scanners are available, a conventional scanner for high quality imaging and a fast resonant scanner for applications such as calcium imaging in living tissue. Two photon SpectraPhysics Mai Tai laser is suitable for imaging of living tissues with greater depth penetration.

Objectives: 10x, 20x, 40x, 63x, 63x (extended focus). One photon excitation lines: 355, 405, 458, 476, 496, 514, 561,633 nm. Two photon excitation: tunable range 690 to 1040 nm.