Reaction: threo-3-hydroxy-L-aspartate = oxaloacetate + NH3
Other name(s): L-threo-3-hydroxyaspartate dehydratase; threo-3-hydroxyaspartate ammonia-lyase
Systematic name: threo-3-hydroxy-L-aspartate ammonia-lyase (oxaloacetate-forming)
Comments: A pyridoxal-phosphate protein. The enzyme, purified from the bacterium Pseudomonas sp. T62, is highly specific, and does not accept any other stereoisomer of 3-hydroxyaspartate. Different from EC 188.8.131.52, erythro-3-hydroxy-L-aspartate ammonia-lyase and EC 184.108.40.206, threo-3-hydroxy-D-aspartate ammonia-lyase. Requires a divalent cation such as Mn2+, Mg2+, or Ca2+.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 248270-70-4
1. Wada, M., Matsumoto, T., Nakamori, S., Sakamoto, M., Kataoka, M., Liu, J.-Q., Itoh, N., Yamada, H. and Shimizu, S. Purification and characterization of a novel enzyme, L-threo-3-hydroxyaspartate dehydratase, from Pseudomonas sp. T62. FEMS Microbiol. Lett. 179 (1999) 147-151. [PMID: 10481099]