Project title: Impact of Ferrodoxin:NADP(H) Reductase localisation in the chloroplast for plant stress tolerance and possible implications in crops improvement
Summary: Ferredoxin:NADP(H) reductase (FNR) is an enzyme involved in photosynthesis and its over-expression confers broad stress tolerance in some plants. The role of FNR in the stress tolerance remains unclear. The localization of FNR in the chloroplast has an influence on electron distribution in photosynthesis and it could also be related to the mechanisms used by plants for developing stress tolerance.
We will investigate how different FNR localisation in the chloroplast affects plant stress tolerance in the model plant Arabidopsis and the crop plant barley. Arabidopsis plants with variable FNR location are already available, and CRISPR-cas9 knockouts in barley of FNR tethering proteins are being developed and screened as part of this project. A better understanding of FNRs role in stressed plants could be highly important for identifying genotypes that help plants to resist adverse conditions.