Grace Heredge Thomas
Project title: Rapid fabrication of custom genome-wide yeast libraries
Summary: Creating a proteome-wide library of tagged proteins has proven immensely useful and has been pioneered in the widely-used model system budding yeast (Saccharomyces cerevisiae). However, creating entire novel libraries is both time consuming and costly.
We propose to create a Rapid Tag Switching (RTS) system to enable researchers to create custom libraries of yeast strains, with each strain encoding a different tagged protein, in as little as one week. Examples of tags could include fluorophores for high-throughput super-resolution imaging, or conditional degrons, which would facilitate study of essential proteins across the genome. For our studies on the kinetochore, we seek to define how specific genetic changes impact upon the process of segregating chromosomes during cell division, as mis-segregated chromosomes are a hallmark of cancer cells. I will use RTS to create libraries of strains that will allow us to quantitatively measure such changes and model the changes seen in cancer cells.