Project title: Characterising the role of kinetochore phosphoregulation
Summary: The kinetochore is a large protein complex that attaches chromosomes to the spindle microtubules during cell division which enables their accurate segregation. Errors in chromosome segregation are a leading cause of birth defects and result in aneuploidy - an incorrect number of chromosomes - which is a hallmark of cancer cells. In yeast, the kinetochore consists of at least 60 different proteins which can occur in multiple copies. Posttranslational modifications of proteins play an essential role in the regulation of the kinetochore and mediate complex assembly and function.
This project aims to analyse the regulation of the kinetochore in budding yeast by posttranslational modifications such as phosphorylation. Using high throughput molecular and cell biology approaches, target sites of specific kinases and phosphatases important for cell cycle regulation will be identified and then genetically manipulated. This allows us to further investigate the effects of protein phosphorylation on the function of the kinetochore using different techniques such as fluorescence microscopy, protein analysis and computational data analysis.