Dr Christoph Engl
Lecturer in Microbiology
Email: email@example.comTelephone: +44 (0)20 7882 3315Room Number: Room 3.06, Fogg building
- 2010 PhD in Biology, Imperial College London, UK
- 2004 German University Diploma in Biology, University of Regensburg, Germany
Awards & Honours
- 2020 Fellow of the Higher Education Academy UK (FHEA)
- 2020 Queen Mary ADEPT Fellow, Queen Mary University of London
- 2015 Queen’s Fellow, Queen’s University Belfast
- 2014 Faculty of Natural Sciences Award for Support of Excellence in Teaching, Imperial College London
- 2005 Faculty of Life Sciences PhD Studentship, Imperial College London
Research in the lab of Dr Engl aims to uncover how bacteria communicate with, adapt to, and shape their environment; with a particular emphasis on Sigma54-regulated systems. We combine cellular, molecular, systems, ecology and state-of-the-art imaging techniques to address fundamental questions in bacterial cell physiology. The overall objective is to advance our understanding in this field to help address major societal challenges such as antimicrobial resistance and sustainability.
Current interests focus on decision-making of individual bacterial cells and on post-transcriptional RNA processing.
Dr Engl is Lecturer in Microbiology. He has been teaching undergraduate and postgraduate students since 2012 and has recently become a Fellow of the Higher Education Academy UK.
Dr Engl received an award for Support for Excellence in Teaching from the Faculty of Life Sciences at Imperial College London and he was nominated by his students for a Student Academic Choice Award from the Imperial College’s Union.
At Queen Mary, Dr Engl teaches on the following modules:
BMD100 Essential Skills for Biomedical Scientists
BMD117 The Microbial World and Humans
BIO231 Microbial Physiology and Growth
BMD600 Research Project
BIO603 Project Skills in the Life Sciences
Alongside his teaching commitments Dr Engl is also:
- Deputy Chair of the Exam Board for UG Biomedical Sciences
- Careers Liaison Tutor for Biomedical Sciences
- Organiser of the Protein & Gene Club (a seminar series for PhD students & PostDocs of the Biochemistry department)
The majority of Dr Engl’s research has centred around adaptive responses involving Sigma54 (s54), a factor that directs RNA polymerase (RNAP) to its cognate promoters. Analogous to eukaryotic systems, Sigma54-dependent transcription initiation strictly requires mechano-chemical energy for the remodelling of the closed to the open promoter complex. This is achieved through ATP hydrolysis by an activator protein that binds to enhancer sequences ~100 nucleotides upstream of the promoter DNA. The enhancer-bound activator is brought into close proximity to the RNAP-s54 holoenzyme through DNA bending via a protein called Integration Host Factor (IHF). The ability of the activator to hydrolyse ATP is tightly regulated. Bacterial cells can encode multiple activators whereby each activator drives the transcription of an adaptive response to a specific stimulus. Sigma54-regulated systems have been identified in the majority of bacterial species. They control important physiological processes including nitrogen fixation and the delivery of virulence factors into host cells by plant and animal pathogens.
Transcription dynamics in individual bacterial cells
One of the best studied Sigma54-regulated systems is the Psp response which maintains proton motive force under membrane stress. Dr Engl has revealed the regulation, spatio-temporal dynamics and stoichiometry of key Psp proteins in live bacterial cells. Recently Dr Engl has utilised the RNA FISH technique to visualise psp mRNA in individual bacterial cells, enabling the determination of the single-cell kinetics and population heterogeneity of Sigma54-regulated transcription. He could demonstrate that the mode of transcriptional bursting is determined by the route to transcription initiation (Engl et al 2020). He could also show that the burst kinetics of Sigma54-regulated promoters resemble those of enhancer-dependent promoters in higher organisms (Engl et al 2020). In collaboration with Prof Mullineaux from the Department of Biochemistry at QMUL, he has further applied RNA FISH to reveal the subcellular localisation of mRNAs encoding proteins of the photosynthetic apparatus in cyanobacteria (Mahbub et al 2020).
Dr Engl currently uses single cell and single molecule fluorescence imaging to study cell-to-cell heterogeneity and population dynamics associated with other molecular switches that drive key adaptive responses in bacteria.
Post-transcriptional RNA processing
Another Sigma54-regulated system studied by Dr Engl is Rtc from E. coli. RtcA (a RNA cyclase) and RtcB (an RNA ligase) encode an RNA-end healing and sealing mechanism that is involved in post-transcriptional RNA processing. Dr Engl could show that the Rtc system helps to maintain the homeostasis of the bacterial ribosome under nutritional stress (Engl et al 2016). The ribosome translates genetic information within mRNA into proteins; it thus provides essential building blocks of the cell. Maintaining ribosome function under fluctuating and challenging environmental conditions is therefore critical for bacteria to thrive. Ribosomes from bacteria and eukaryotes differ, enabling targeted interference with ribosome function as a means to halt bacterial growth. Hence, the ribosome is the target of the majority of antimicrobials currently used in clinical and agricultural settings. Strikingly, the Rtc system is induced by and increases the tolerance to antimicrobials that specifically target the ribosome (Engl et al 2016). As such, Rtc represents a physiological response to antimicrobials that was previously overlooked. How it increases the tolerance to ribosome targeting antimicrobials is currently unknown. Dr Engl is therefore continuing his investigation into post-transcriptional RNA processing by Rtc.
Research in the Engl lab is funded by grants from the Wellcome Trust and the Leverhulme Trust.
Engl C*, Jovanovic G, Brackston RD, Kotta-Loizou I, Buck M (2020) The route to transcription initiation determines the mode of transcriptional bursting in bacteria. Nat Commun 11: 1-11.
Mahbub M, Hemm L, Yang Y, Kaur R, Carmen H, Engl C, Huokko T, Riediger M, Watanabe S, Liu L-N, Wilde A, Hess W, Mullineaux CW (2020) mRNA localisation, reaction centre biogenesis and thylakoid membrane targeting in cyanobacteria. Nat Plants 6: 1179-1191.
Engl C* (2019) Noise in bacterial gene expression. Biochem Soc Trans 47: 209-217.
Finch EA, Caruso T, Engl C* (2018) Effects of Paenibacillus polymyxa inoculation on below-ground nematode communities and plant growth. Soil Biol Biochem 121: 1-7.
Engl C*, Schaefer J, Kotta-Loizou I, Buck M. (2016) Cellular and molecular phenotypes depending upon the RNA repair system RtcAB in Escherichia coli. Nucleic Acids Res 44: 9933-9941.
Schaefer J, Engl C*, Zhang N, Lawton E, Buck M (2015) Genome wide interactions of wild-type and activator bypass forms of s54. Nucleic Acids Res 43: 7280-91.
Engl C*, Waite CJ, McKenna JF, Bennett MH, Hamann T, Buck M (2014) Chp8, a diguanylate cyclase from Pseudomonas syringae pv tomato DC3000 suppresses the PAMP flagellin, increases EPS and promotes plant immune evasion. mBio 5: e01168-14.
Browse a list of publications by Christoph Eng
Katherine Fenn Research Technician on the Wellcome Trust grant (Ribosomes under Attack).
Current PhD students:
Current open positions:
- A PostDoc and a PhD position are available on the Leverhulme Trust funded project: Bacterial decision-making: Exploring molecular switches of population dynamics
In this project we will investigate how decision-making works at the single-cell level, and how bacterial populations move from individual to group behaviour. To do this we will combine molecular genetics (to dissect signalling and gene-expression pathways), next generation sequencing (to capture the process of transcription and translation with single codon-resolution), fluorescence imaging (to determine the number of mRNA molecules in individual cells across bacterial populations) and mathematical modelling (to extract from our data the dynamics of decision-making and make predictions for expected outcomes given different environmental conditions). Ultimately, deconstructing the decision-making process means that we can manipulate bacteria to adopt a behaviour that is more efficient for the intended purpose, be it commercial (e.g. in the food industry or chemical manufacturing) or clinical (e.g. when treating bacterial infections).
For more information on the scientific background and the methodology please see:
Engl 2019 Biochem Soc Trans 47:209-217 (doi: 10.1042/BST20180500)
Engl et al 2020 Nature Commun 11:2422 (doi: 10.1038/s41467-020-16367-6).
Interested candidates are welcome to contact Dr. Christoph Engl at firstname.lastname@example.org. Please include your CV, motivation letter and contact details of two academic referees.
Dr Engl co-organised the following public event:
Microbes: The Good, the Bad and the Ugly (with Dr Adam Rutherford & Guests)
Public lectures on how microorganisms change our world
Northern Ireland Science Festival 2017
Audience >100, Age 14+