Reaction: 2,4-dichloro-2,5-dihydro-5-oxofuran-2-acetate = 2,4-dichloro-cis,cis-muconate
For diagram click here.
Other name(s): α-pinene oxide lyase; α-pinene-oxide lyase (decyclizing)
Systematic name: α-pinene-oxide lyase (ring-opening)
Comments: Requires Mn2+. The product of cycloisomerization of dichloro-cis,cis-muconate spontaneously eliminates chloride to produce cis-4-carboxymethylene-3-chlorobut-2-en-4-olide. Also acts, in the reverse direction, on cis,cis-muconate and its monochloro-derivatives, but with lower affinity. Not identical with EC 22.214.171.124 (muconate cycloisomerase) or EC 126.96.36.199 (chloromuconate cycloisomerase).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 126904-95-8
1. Kuhm, A.E., Schlömann, M., Knackmuss, H.-J. and Pieper, D.H. Purification and characterization of dichloromuconate cycloisomerase from Alcaligenes eutrophus JMP 134. Biochem. J. 266 (1990) 877-883. [PMID: 2327971]