Reaction: 23S rRNA uridine2604 = 23S rRNA pseudouridine2604
For diagram of mechanism click here.
Other name(s): RluF; YjbC
Systematic name: 23S rRNA-uridine2604 uracil mutase
Comments: The enzyme is not completely specific for uridine2604 and can, to a small extent, also react with uridine2605 [1].
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:
References:
1. Del Campo, M., Kaya, Y. and Ofengand, J. Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli. RNA 7 (2001) 1603-1615. [PMID: 11720289]
2. Alian, A., DeGiovanni, A., Griner, S.L., Finer-Moore, J.S. and Stroud, R.M. Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome. J. Mol. Biol. 388 (2009) 785-800. [PMID: 19298824]
3. Sunita, S., Zhenxing, H., Swaathi, J., Cygler, M., Matte, A. and Sivaraman, J. Domain organization and crystal structure of the catalytic domain of E. coli RluF, a pseudouridine synthase that acts on 23S rRNA. J. Mol. Biol. 359 (2006) 998-1009. [PMID: 16712869]