Reaction: S-(hydroxymethyl)glutathione = glutathione + formaldehyde
Other name(s): glutathione-dependent formaldehyde-activating enzyme; Gfa; S-(hydroxymethyl)glutathione formaldehyde-lyase
Systematic name: S-(hydroxymethyl)glutathione formaldehyde-lyase (glutathione-forming)
Comments: The enzyme from Paracoccus denitrificans accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 22.214.171.1244, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, UM-BBD, CAS registry number: 425642-27-9
1. Goenrich, M., Bartoschek, S., Hagemeier, C.H., Griesinger, C. and Vorholt, J.A. A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy. J. Biol. Chem. 277 (2002) 3069-3072. [PMID: 11741920]