Reaction: (2E,6E)-farnesyl diphosphate + H2O = kunzeaol + diphosphate
For diagram of reaction click here.
Glossary: kunzeaol = 6β-hydroxygermacra-1(10),4-diene = (1R,2E,6E,10R)-3,7-dimethyl-10-isopropylcyclodeca-2,6-dienol
Other name(s): TgTPS2 (gene name)
Systematic name: (2E,6E)-farnesyl-diphosphate diphosphate-lyase (kunzeaol forming)
Comments: Isolated from the root of the plant Thapsia garganica. The enzyme also produces germacrene D, bicyclogermacrene and traces of other sesquiterpenoids. See EC 220.127.116.11, (+)-germacrene D synthase and EC 18.104.22.168, bicyclogermacrene synthase.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Pickel, B., Drew, D.P., Manczak, T., Weitzel, C., Simonsen, H.T. and Ro, D.K. Identification and characterization of a kunzeaol synthase from Thapsia garganica: implications for the biosynthesis of the pharmaceutical thapsigargin. Biochem. J. 448 (2012) 261-271. [PMID: 22938155]