Reaction: N-cyclohexylformamide = cyclohexyl isocyanide + H2O
Other name(s): isonitrile hydratase; N-cyclohexylformamide hydro-lyase
Systematic name: N-cyclohexylformamide hydro-lyase (cyclohexyl-isocyanide-forming)
Comments: The enzyme from Pseudomonas putida strain N19-2 can also catalyse the hydration of other isonitriles to the corresponding N-substituted formamides. The enzyme has no metal requirements.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 358974-06-8
1. Goda, M., Hashimoto, Y., Shimizu, S. and Kobayashi, M. Discovery of a novel enzyme, isonitrile hydratase, involved in nitrogen-carbon triple bond cleavage. J. Biol. Chem. 276 (2001) 23480-23485. [PMID: 11306561]