Reaction: a [lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine + H2O = a [lipoyl-carrier protein]-L-lysine + (R)-lipoate
Other name(s): pyruvate dehydrogenase inactivase
Systematic name: [lipoyl-carrier protein]-N6-[(R)-lipoyl]-L-lysine amidohydrolase
Comments: The enzyme, characterized from the bacterium Enterococcus faecalis, is a member of the Ser-Ser-Lys triad amidohydrolase family. cf. EC 2.3.1.313, NAD-dependent lipoamidase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Reed, L.J., Koike, M., Levitch, M.E., Leach, F.R. Studies on the nature and reactions of protein-bound lipoic acid. J. Biol. Chem. 232 (1958) 143-158. [PMID: 13549405]
2. Suzuki, K, Reed L.J. Lipoamidase. J. Biol. Chem. 238 (1963) 4021-4025. [PMID: 14086741]
3. Jiang, Y. and Cronan, J.E. Expression cloning and demonstration of Enterococcus faecalis lipoamidase (pyruvate dehydrogenase inactivase) as a Ser-Ser-Lys triad amidohydrolase. J. Biol. Chem. 280 (2005) 2244-2256. [PMID: 15528186]