Arg-Phe-Ala
Reaction: Cleavage of the proline- and hydroxyproline-rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, gelatin and Leu-Trp-MetArg-Phe-Ala
Other names: autolysin; Chlamydomonas cell wall degrading protease; lysin; Chlamydomonas reinhardtii metalloproteinase; gamete lytic enzyme; gamete autolysin
Comments: A glycoprotein found in the periplasmic space of Chlamydomonas reinhardtii gametes in a 62 kDa inactive form; decreased to 60 kDa upon activation. A zinc enzyme, inhibited by phosphoramidon, but also thiol activated. Type example of peptidase family M11
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, CAS registry number: 97089-74-2
References:
1. Jaenicke, L., Kunhe, W., Spessert, R., Wahle, U. and Waffenschmidt, S. Cell-wall lytic enzymes (autolysins) of Chlamydomonas reinhardtii are (hydroxy)proline-specific proteases. Eur. J. Biochem. 170 (1987) 485-491. [PMID: 3319620]
2. Buchanan, M.J., Imam, S.H., Eskue, W.A. and Snell, W.J. Activation of the cell wall degrading protease, lysin, during sexual signalling in Chlamydomonas: the enzyme is stored as an inactive, higher relative molecular mass precursor in the periplasm. J. Cell. Biol. 108 (1989) 199-207. [PMID: 2910877]
3. Matsuda, Y. Gametolysin. In: Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D. & Woessner, J.F. eds), pp. 1140-1143 (1998) Academic Press, London