Val bond in plasminogen to form plasmin
Reaction: Specific cleavage of ArgVal bond in plasminogen to form plasmin
Other names: tissue plasminogen activator; plasminogen activator, tissue-type; tissue-type plasminogen activator; tPA; t-PA
Comments: A peptidase of family S1 (trypsin family) from a wide variety of mammalian tissues, especially endothelial cells. Secreted as a single chain precursor which is cleaved to a two-chain form by plasmin. Activity is considerably enhanced by fibrin. Formerly included in EC 3.4.21.31 and EC 3.4.99.26
Links to other databases: BRENDA, EXPASY, KEGG, MEROPS, Metacyc, PDB, CAS registry number: 139639-23-9
References
1. Pennica, D., Holmes, W.E., Kohr, W.J., Harkins, R.N., Vehar, G.A., Ward, C.A., Bennett, W.F., Yelverton, E., Seeburg, P.H., Heyneker, H.L., Goeddel, D.V. and Collen, D. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 301 (1983) 214-221. [PMID: 6337343]
2. Loskutoff, D.J. and Schleef, R.R. Plasminogen activators and their inhibitors. Methods Enzymol. 163 (1988) 293-302. [PMID: 3148826]
3. Petersen, L.C., Johannessen, M., Forster, D., Kumar, A. and Mulvihill, E. The effect of polymerised fibrin on the catalytic activities of one-chain tissue-type plasminogen activator as revealed by an analogue resistant to plasmin cleavage. Biochim. Biophys. Acta 952 (1988) 245-254 [PMID: 2962643]
4. Verheijen, J.H. Tissue-type plasminogen activator and fast-acting plasminogen activator inhibitor in plasma. Methods Enzymol. 163 (1988) 302-309. [PMID: 3148827]
5. Gerard, R.D. and Meidell, R.S. Regulation of tissue plasminogen activator expression. Annu. Rev. Physiol. 51 (1989) 245-262. [PMID: 2496643]
6. Collen, D., Lijnen, H. R. and Verstraete, M. The fibrinolytic system and its disorders. In Blood: Principles and Practice of Hematology, 2nd edn (Handin, R.I., Lux, S.E. and Stossel, J.P., eds), J.B.Lippincott Company, Philadelphia (1990)