Reaction: 2,4-dinitroanisole + H2O = methanol + 2,4-dinitrophenol
Glossary: 2,4-dinitroanisole = 1-methoxy-2,4-dinitrobenzene
Other name(s): 2,4-dinitroanisole ether hydrolase; dnhA (gene name); dnhB (gene name); DNAN demethylase
Systematic name: 2,4-dinitroanisole methanol hydrolase
Comments: The enzyme, characterized from the bacterium Nocardioides sp. JS1661, is involved in the degradation of 2,4-dinitroanisole. Unlike other known O-demethylases, such as EC 184.108.40.206, 4-methoxybenzoate monooxygenase (O-demethylating), or EC 220.127.116.11, codeine 3-O-demethylase, it does not require oxygen or electron donors, and produces methanol rather than formaldehyde.
1. Fida, T.T., Palamuru, S., Pandey, G. and Spain, J.C. Aerobic biodegradation of 2,4-dinitroanisole by Nocardioides sp. strain JS1661. Appl. Environ. Microbiol. 80 (2014) 7725-7731. [PMID: 25281383]