Reaction: Endohydrolysis of 3-O-methyl-α-D-mannosyl-(1→4)-3-O-methyl-D-mannose linkages within (1,4)-3-O-methyl-α-D-mannnan substrates
Glossary: MMP = 3-O-methylmannose polysaccharide = α-D-mannosyl-(1→4)-[3-O-methyl-α-D-mannosyl-(1→4)]n-1-O,3-O-dimethyl-α-D-mannose
Other name(s): MMP α-(1→4)-endomannosidase; mmpH (gene name)
Systematic name: (1,4)-3-O-methyl-α-D-mannan 4-α-3-O-methyl-D-mannohydrolase
Comments: The enzyme, present in mycobacterial species that produce 3-O-methylmannose polysaccharide (MMP), is involved in recycling and biosynthesis of the polymer. The enzyme has been shown to cleave substrates in the range of 11-14 mannose residues.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Maranha, A., Costa, M., Ripoll-Rozada, J., Manso, J.A., Miranda, V., Mendes, V.M., Manadas, B., Macedo-Ribeiro, S., Ventura, M.R., Pereira, P.JB. and Empadinhas, N. Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides. Commun Biol 6 (2023) 108. [PMID: 36707645]