Reaction: kojibiose + H2O = β-D-glucopyranose + D-glucopyranose
Glossary: kojibiose = α-D-glucopyranosyl-(1→2)-D-glucopyranose
Other name(s): kojibiase
Systematic name: kojibiose glucohydrolase (configuration-inverting)
Comments: The enzyme, characterized from the bacteria Flavobacterium johnsoniae and Mucilaginibacter mallensis, uses anomer-inverting mechanism to release β-glucose from the non-reducing ends of kojibiose and α-1,2-oligoglucans with a higher degree of polymerization.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Nakamura, S., Nihira, T., Kurata, R., Nakai, H., Funane, K., Park, E.Y. and Miyazaki, T. Structure of a bacterial α-1,2-glucosidase defines mechanisms of hydrolysis and substrate specificity in GH65 family hydrolases. J. Biol. Chem. (2021) 101366. [PMID: 34728215]
2. De Beul, E., Jongbloet, A., Franceus, J. and Desmet, T. Discovery of a kojibiose hydrolase by analysis of specificity-determining correlated positions in glycoside hydrolase family 65. Molecules 26 (2021) 6321. [PMID: 34684901]