Reaction: isomaltose + H2O = β-D-glucose + D-glucose
Systematic name: isomaltose 6-α-glucohydrolase (configuration-inverting)
Comments: The enzyme catalyses the hydrolysis of α-1,6-glucosidic linkages from the non-reducing end of its substrate. Unlike EC 18.104.22.168, oligo-1,6-glucosidase, the enzyme inverts the anomeric configuration of the released residue. The enzyme can also act on panose and maltotriose at a lower rate.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Tagami, T., Miyano, E., Sadahiro, J., Okuyama, M., Iwasaki, T. and Kimura, A. Two novel glycoside hydrolases responsible for the catabolism of cyclobis-(1→6)-α-nigerosyl. J. Biol. Chem. 291 (2016) 16438-16447. [PMID: 27302067]