Reaction: 3-methylbutyl acetate + H2O = 3-methylbutanol + acetate
Other name(s): IAH1 (gene name)
Systematic name: 3-methylbutyl acetate acetohydrolase
Comments: The enzyme, characterized from the yeast Saccharomyces cerevisiae, hydrolyses acetate esters. It acts preferentially on 3-methylbutyl acetate, a major determinant of sake flavor.
1. Fukuda, K., Kiyokawa, Y., Yanagiuchi, T., Wakai, Y., Kitamoto, K., Inoue, Y. and Kimura, A. Purification and characterization of isoamyl acetate-hydrolyzing esterase encoded by the IAH1 gene of Saccharomyces cerevisiae from a recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 53 (2000) 596-600. [PMID: 10855721]