Reaction: acetyl-CoA + malonyl-CoA = acetoacetyl-CoA + CoA + CO2
Other name(s): NphT7
Systematic name: acetyl-CoA:malonyl-CoA C-acetyltransferase (decarboxylating)
Comments: The enzyme from the soil bacterium Streptomyces sp. CL190 produces acetoacetyl-CoA to be used for mevalonate production via the mevalonate pathway. Unlike the homologous EC 22.214.171.124 (β-ketoacyl-[acyl-carrier-protein] synthase III), this enzyme does not accept malonyl-[acyl-carrier-protein] as a substrate.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Okamura, E., Tomita, T., Sawa, R., Nishiyama, M. and Kuzuyama, T. Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway. Proc. Natl. Acad. Sci. USA 107 (2010) 11265-11270. [PMID: 20534558]