Reaction: an acyl-CoA + pseudotropine = CoA + an O-acylpseudotropine
For diagram of reaction, click here
Glossary: tropine = tropan-3β-ol = 3β-hydroxytropane
Other name(s): pseudotropine:acyl-CoA transferase; tigloyl-CoA:pseudotropine acyltransferase; acetyl-CoA:pseudotropine acyltransferase; pseudotropine acetyltransferase; pseudotropine tigloyltransferase; PAT (ambiguous)
Systematic name: acyl-CoA:pseudotropine O-acyltransferase
Comments: This enzyme exhibits absolute specificity for the exo/3β configuration found in pseudotropine as tropine (tropan-3α-ol; see EC 220.127.116.11, tropine acyltransferase) and nortropine are not substrates . Acts on a wide range of aliphatic acyl-CoA derivatives, including acetyl-CoA, β-methylcrotonyl-CoA and tigloyl-CoA .
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Rabot, S., Peerless, A.C.J. and Robins, R.J. Tigloyl-CoA:pseudotropine acyltransferase an enzyme of tropane alkaloid biosynthesis. Phytochemistry 39 (1995) 315-322.
2. Robins, R.J., Bachmann, P., Robinson, T., Rhodes, M.J. and Yamada, Y. The formation of 3α- and 3β-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases. FEBS Lett. 292 (1991) 293-297. [PMID: 1959620]
3. Robins, R.J., Bachmann,P., Peerless, A.C.J. and Rabot, S. Esterification reactions in the biosynthesis of tropane alkaloids in transformed root cultures. Plant Cell, Tissue Organ Cult. 38 (1994) 241-247.
4. Boswell, H.D., Dräger, B., McLauchlan, W.R., Portsteffen, A., Robins, D.J., Robins, R.J. and Walton, N.J. Specificities of the enzymes of N-alkyltropane biosynthesis in Brugmansia and Datura. Phytochemistry 52 (1999) 871-878. [PMID: 10626376]