EC 2.1.1.187

Reaction: S-adenosyl-L-methionine + guanine⁷⁴⁵ in 23S rRNA = S-adenosyl-L-homocysteine + N¹-methylguanine⁷⁴⁵ in 23S rRNA

Other name(s): Rlma(I); Rlma1; 23S rRNA m¹G⁷⁴⁵ methyltransferase; YebH; RlmA^I methyltransferase; ribosomal RNA(m¹G)-methylase (ambiguous); rRNA(m¹G)methylase (ambiguous); RrmA (ambiguous); 23S rRNA:m¹G⁷⁴⁵ methyltransferase

Systematic name: S-adenosyl-L-methionine:23S rRNA (guanine⁷⁴⁵-N¹)-methyltransferase

Comments: The enzyme specifically methylates guanine⁷⁴⁵ at N¹ in 23S rRNA.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:

References:

1. Liu, M., Novotny, G.W. and Douthwaite, S. Methylation of 23S rRNA nucleotide G⁷⁴⁵ is a secondary function of the RlmA^I methyltransferase. RNA 10 (2004) 1713-1720. [PMID: 15388872]

2. Gustafsson, C. and Persson, B.C. Identification of the rrmA gene encoding the 23S rRNA m¹G⁷⁴⁵ methyltransferase in Escherichia coli and characterization of an m¹G⁷⁴⁵-deficient mutant. J. Bacteriol. 180 (1998) 359-365. [PMID: 9440525]

3. Das, K., Acton, T., Chiang, Y., Shih, L., Arnold, E. and Montelione, G.T. Crystal structure of RlmA^I: implications for understanding the 23S rRNA G⁷⁴⁵/G⁷⁴⁸-methylation at the macrolide antibiotic-binding site. Proc. Natl. Acad. Sci. USA 101 (2004) 4041-4046. [PMID: 14999102]

4. Hansen, L.H., Kirpekar, F. and Douthwaite, S. Recognition of nucleotide G⁷⁴⁵ in 23 S ribosomal RNA by the rrmA methyltransferase. J. Mol. Biol. 310 (2001) 1001-1010. [PMID: 11501991]

5. Liu, M. and Douthwaite, S. Methylation at nucleotide G⁷⁴⁵ or G⁷⁴⁸ in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria. Mol. Microbiol. 44 (2002) 195-204. [PMID: 11967079]