EC 1.3.3.8

Reaction: (S)-tetrahydroberberine + 2 O₂ = berberine + 2 H₂O₂

For diagram click here or here.

Other name(s): (S)-THB oxidase

Systematic name: (S)-tetrahydroberberine:oxygen oxidoreductase

Comments: The enzyme from Berberis sp. (previously listed as EC 1.5.3.8) is a flavoprotein; that from Coptis japonica is not. (R)-Tetrahydroberberines are not oxidized.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 114705-00-9

References:

1. Amann, M., Nagakura, N. and Zenk, M.H. (S)-Tetrahydroprotoberberine oxidase the final enzyme in protoberberine biosynthesis. Tetrahedron Lett. 25 (1984) 953-954.

2. Okada, N., Shinmyo, A., Okada, H. and Yamada, Y. Purification and characterization of (S)-tetrahydroberberine oxidase from cultured Coptis japonica cells. Phytochemistry 27 (1988) 979-982.