Reaction: 7β-hydroxy-3-oxochol-24-oyl-CoA + NAD+ = 7β-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
Other name(s): baiH (gene name)
Systematic name: 7β-hydroxy-3-oxochol-24-oyl-CoA Δ4-oxidoreductase
Comments: Contains FAD and FMN. The enzyme, characterized from the bacterium Clostridium scindens, participates in the bile acid 7α-dehydroxylation pathway. The enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7α anomer. cf. EC 18.104.22.168, 3-oxocholoyl-CoA 4-desaturase.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Baron, S.F. and Hylemon, P.B. Expression of the bile acid-inducible NADH:flavin oxidoreductase gene of Eubacterium sp. VPI 12708 in Escherichia coli. Biochim. Biophys. Acta 1249 (1995) 145-154. [PMID: 7599167]
2. Franklund, C.V., Baron, S.F. and Hylemon, P.B. Characterization of the baiH gene encoding a bile acid-inducible NADH:flavin oxidoreductase from Eubacterium sp. strain VPI 12708. J. Bacteriol. 175 (1993) 3002-3012. [PMID: 8491719]
3. Kang, D.J., Ridlon, J.M., Moore, D.R., 2nd, Barnes, S. and Hylemon, P.B. Clostridium scindens baiCD and baiH genes encode stereo-specific 7α/7β-hydroxy-3-oxo-Δ4-cholenoic acid oxidoreductases. Biochim. Biophys. Acta 1781 (2008) 16-25. [PMID: 18047844]