Reaction: formate + NAD+ = CO2 + NADH
Other name(s): formate-NAD+ oxidoreductase; FDH I; FDH II; N-FDH; formic hydrogen-lyase; formate hydrogenlyase; hydrogenlyase; NAD+-linked formate dehydrogenase; NAD+-dependent formate dehydrogenase; formate dehydrogenase (NAD+); NAD+-formate dehydrogenase; formate benzyl-viologen oxidoreductase; formic acid dehydrogenase
Systematic name: formate:NAD+ oxidoreductase
Comments: The enzyme from most aerobic organisms is devoid of redox-active centres but that from the proteobacterium Methylosinus trichosporium contains iron-sulfur centres, flavin and a molybdenum centre . Together with EC 220.127.116.11 hydrogen dehydrogenase, forms a system previously known as formate hydrogenlyase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Davison, D.C. Studies on plant formic dehydrogenase. Biochem. J. 49 (1951) 520-526. [PMID: 14886318]
2. Quayle, J.R. Formate dehydrogenase. Methods Enzymol. 9 (1966) 360-364.
3. Jollie, D.R. and Lipscomb, J.D. Formate dehydrogenase from Methylosinus trichosporium OB3b. Purification and spectroscopic characterization of the cofactors. J. Biol. Chem. 266 (1991) 21853-21863. [PMID: 1657982]