IUBMB Enzyme Nomenclature

EC 1.14.99.37

Accepted name: taxadiene 5α-hydroxylase

Reaction: taxa-4,11-diene + reduced acceptor + O2 = taxa-4(20),11-dien-5α-ol + acceptor + H2O

For diagram click here.

Systematic name: taxa-4,11-diene,hydrogen-donor:oxygen oxidoreductase (5α-hydroxylating)

Comments: This microsomal cytochrome-P450-dependent enzyme is involved in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel). The reaction includes rearrangement of the 4(5)-double bond to a 4(20)-double bond, possibly through allylic oxidation.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 9035-51-2

References:

1. Hefner, J., Rubenstein, S.M., Ketchum, R.E., Gibson, D.M., Williams, R.M. and Croteau, R. Cytochrome P450-catalyzed hydroxylation of taxa-4(5),11(12)-diene to taxa-4(20),11(12)-dien-5alpha-ol: the first oxygenation step in taxol biosynthesis. Chem. Biol. 3 (1996) 479-489. [PMID: 8807878]

[EC 1.14.99.37 created 2002]


Return to EC 1.14.99 home page
Return to EC 1.14 home page
Return to EC 1 home page
Return to Enzymes home page
Return to IUBMB Biochemical Nomenclature home page