Reaction: ent-kaur-16-ene + 3 [reduced NADPHhemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPHhemoprotein reductase] + 4 H2O (overall reaction)
(1a) ent-kaur-16-ene + [reduced NADPHhemoprotein reductase] + O2 = ent-kaur-16-en-19-ol + [oxidized NADPHhemoprotein reductase] + H2O
(1b) ent-kaur-16-en-19-ol + [reduced NADPHhemoprotein reductase] + O2 = ent-kaur-16-en-19-al + [oxidized NADPHhemoprotein reductase] + 2 H2O
(1c) ent-kaur-16-en-19-al + [reduced NADPHhemoprotein reductase] + O2 = ent-kaur-16-en-19-oate + [oxidized NADPHhemoprotein reductase] + H2O
For diagram of reaction click here.
Other name(s): ent-kaurene oxidase (misleading)
Systematic name: ent-kaur-16-ene,[reduced NADPHhemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
Comments: A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Ashman, P.J., Mackenzie, A. and Bramley, P.M. Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi. Biochim. Biophys. Acta 1036 (1990) 151-157. [PMID: 2223832]
2. Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima. Biochem. Soc. Trans. 20 (1992) 218. [PMID: 1397591]
3. Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis. Plant Physiol. 119 (1999) 507-510. [PMID: 9952446]