Reaction: ent-isokaurene + 2 O2 + 2 [reduced NADPHhemoprotein reductase] = ent-isokaurene-2β,3β-diol + [oxidized NADPHhemoprotein reductase] + 2 H2O (overall reaction)
(1a) ent-isokaurene + O2 + [reduced NADPHhemoprotein reductase] = ent-isokauren-2β-ol + [oxidized NADPHhemoprotein reductase] + H2O
(1b) ent-isokauren-2β-ol + O2 + [reduced NADPHhemoprotein reductase] = ent-isokaurene-2β,3β-diol + [oxidized NADPHhemoprotein reductase] + H2O
For diagram of reaction click here.
Other name(s): CYP71Z6; ent-isokaurene C2-hydroxylase
Systematic name: ent-isokaurene,[reduced NADPHhemoprotein reductase]:oxygen oxidoreductase (ent-isokaurene-2β,3β-diol forming)
Comments: This cytochrome P-450 (heme thiolate) enzyme has been characterized from the plant Oryza sativa (rice). It may be involved in production of oryzadione.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Wu, Y., Hillwig, M.L., Wang, Q. and Peters, R.J. Parsing a multifunctional biosynthetic gene cluster from rice: biochemical characterization of CYP71Z6 & 7. FEBS Lett. 585 (2011) 3446-3451. [PMID: 21985968]
2. Kitaoka, N., Wu, Y., Xu, M. and Peters, R.J. Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis. Appl. Microbiol. Biotechnol. 99 (2015) 7549-7558. [PMID: 25758958]