Reaction: sophoraflavanone B + [reduced NADPHhemoprotein reductase] + O2 = leachianone G + [oxidized NADPHhemoprotein reductase] + H2O
For diagram of reaction click here.
Glossary: sophoraflavanone B = ()-(2S)-8-dimethylallylnaringenin = ()-(2S)-5,7-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methylbut-2-enyl)chroman-4-one
leachianone G = ()-(2S)-2'-hydroxy-8-dimethylallylnaringenin = ()-(2S)-5,7-dihydroxy-8-(2-hydroxy-3-methylbut-2-enyl)-2-(4-hydroxyphenyl)chroman-4-one
Other name(s): 8-DMAN 2'-hydroxylase
Systematic name: sophoraflavanone-B,[reduced NADPHhemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
Comments: A membrane-bound cytochrome P-450 (heme-thiolate) protein that is associated with the endoplasmic reticulum [1,2]. This enzyme is specific for sophoraflavanone B as substrate. Along with EC 18.104.22.168 (naringenin 8-dimethylallyltransferase) and EC 22.214.171.124 (leachianone G 2''-dimethylallyltransferase), this enzyme forms part of the sophoraflavanone G biosynthetic pathway.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Yamamoto, H., Yatou, A. and Inoue, K. 8-Dimethylallylnaringenin 2'-hydroxylase, the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells. Phytochemistry 58 (2001) 671-676. [PMID: 11672730]
2. Zhao, P., Inoue, K., Kouno, I. and Yamamoto, H. Characterization of leachianone G 2''-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures. Plant Physiol. 133 (2003) 1306-1313. [PMID: 14551337]