Reaction: 3-(2-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O
Other name(s): 2-hydroxyphenylpropionate hydroxylase; melilotate hydroxylase; 2-hydroxyphenylpropionic hydroxylase; melilotic hydroxylase
Systematic name: 3-(2-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (3-hydroxylating)
Comments: A flavoprotein (FAD).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 37256-72-7
References:
1. Levy, C.C. Melilotate hydroxylase. Purification of the enzyme and the nature of the prosthetic group. J. Biol. Chem. 242 (1967) 747-753. [PMID: 6017743]
2. Levy, C.C. and Frost, P. The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase. J. Biol. Chem. 241 (1966) 997-1003. [PMID: 4285850]
3. Strickland, S. and Massey, V. The purification and properties of the flavoprotein melilotate hydroxylase. J. Biol. Chem. 248 (1973) 2944-2952. [PMID: 4348920]
4. Strickland, S. and Massey, V. The mechanism of action of the flavoprotein melilotate hydroxylase. J. Biol. Chem. 248 (1973) 2953-2962. [PMID: 4348921]