Reaction: (indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O
Glossary: (indol-3-yl)acetate = (1H-indol-3-yl)acetate = indole-3-acetate
Other name(s): iacA (gene name)
Systematic name: (indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating)
Comments: The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Leveau, J.H. and Lindow, S.E. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl. Environ. Microbiol. 71 (2005) 2365-2371. [PMID: 15870323]
2. Scott, J.C., Greenhut, I.V. and Leveau, J.H. Functional characterization of the bacterial iac genes for degradation of the plant hormone indole-3-acetic acid. J Chem Ecol 39 (2013) 942-951. [PMID: 23881445]