Reaction: protoasukamycin + NADH + H+ + O2 = 4-hydroxyprotoasukamycin + NAD+ + H2O
Glossary: asuE1 (gene name)
Systematic name: protoasukamycin,NADH:oxygen oxidoreductase (4-hydroxylating)
Comments: The enzyme, characterized from the bacterium Streptomyces nodosus subsp. asukaensis, is involved in the biosynthesis of the antibiotic asukamycin. Requires a flavin cofactor, with no preference among FMN, FAD or riboflavin. When flavin concentration is low, activity is enhanced by the presence of the NADH-dependent flavin-reductase AsuE2.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Rui, Z., Sandy, M., Jung, B. and Zhang, W. Tandem enzymatic oxygenations in biosynthesis of epoxyquinone pharmacophore of manumycin-type metabolites. Chem. Biol. 20 (2013) 879-887. [PMID: 23890006]