Reaction: D-mannitol + O2 = D-mannose + H2O2
Other name(s): mannitol oxidase; D-arabitol oxidase
Systematic name: mannitol:oxygen oxidoreductase (cyclizing)
Comments: Also catalyses the oxidation of D-arabinitol and, to a lesser extent, D-glucitol (sorbitol), whereas L-arabinitol is not a good substrate. The enzyme from the snails Helix aspersa and Arion ater is found in a specialised tubular organelle that has been termed the mannosome.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 73562-29-5
References:
1. Vorhaben, J.E., Scott, J.F., Smith, D.D. and Campbell, J.W. Mannitol oxidase: partial purification and characterisation of the membrane-bound enzyme from the snail Helix aspersa. Int. J. Biochem. 18 (1986) 337-344. [PMID: 3519307]
2. Malik, Z., Jones, C.J.P. and Connock, M.J. Assay and subcellular localization of H2O2 generating mannitol oxidase in the terrestrial slug Arion ater. J. Exp. Zool. 242 (1987) 9-15.
3. Large, A.T., Jones, C.J.P. and Connock, M.J. The association of mannitol oxidase with a distinct organelle in the digestive gland of the terrestrial slug Arion ater. Protoplasma 175 (1993) 93-101.
4. Lobo-da-Cunha, A., Amaral-de-Carvalho, D., Oliveira, E., Alves, A., Costa, V. and Calado, G. Mannitol oxidase and polyol dehydrogenases in the digestive gland of gastropods: Correlations with phylogeny and diet. PLoS One 13 (2018) e0193078. [PMID: 29529078]