Reaction: 3α,7α,12β-trihydroxy-5β-cholan-24-oate + NADP+ = 3α,7α-dihydroxy-12-oxo-5β-cholan-24-oate + NADPH + H+
Other name(s): 12β-hydroxy steroid (nicotinamide adenine dinucleotide phosphate) dehydrogenase
Systematic name: 12β-hydroxysteroid:NADP+ 12-oxidoreductase
Comments: Acts on a number of bile acids, both in their free and conjugated forms.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 118390-62-8
1. Edenharder, R. and Pfützner, A. Characterization of NADP-dependent 12β-hydroxysteroid dehydrogenase from Clostridium paraputrificum. Biochim. Biophys. Acta 962 (1988) 362-370. [PMID: 3167086]