At Mile End we can grow up to >30 x 2L flasks at any one time, at 4 different temperatures.
At the Blizard we can grow up to 16 x 2L flasks at any one time, at 2 different temperatures.
Training and booking is supported by Dr. Petra Ungerer - p.ungerer@qmul.ac.uk
The vibraphonics ultrasonic processor is perfect for lysing smaller volumes of cells.
We have two probes allowing effective lysis of samples for 1ml-200ml. Access is granted by the facility.
The LM20 allows complete lysis of samples from 10 ml-1L. Our model operates pressures up to 30,000 psi (2068 bar) allowing the lysis of cells that are typically more difficult to disrupt such as yeast and diatoms. The cooling system and high pressure facilitates extremely efficient lysis with minimum impact on the proteins of interest.
We’re so happy with this piece of kit Analytik filmed us using it.
At SBBS, we have 2 standard high speed Beckman centrifuges (Avanti 30i) and an ultracentrifuge (Beckman Optima L-80XP) with rotors capable of separating the following:
Proteins are involved in a wide variety of processes. Depending on your requirements, we have a wide variety of technologies and skills that will allow you to obtain protein suitable for your needs.
We have expertise in the purification of antibodies for western blotting and proteins destined for crystallisation, where a highly homogenous sample is required.
We have a wide variety of equipment and expertise in the purification of proteins. To facilitate this we have 3 AKTA systems, one maintained at 4C allowing flexibility and reliable, high performance protein purification.
Training is compulsory and managed by Dr Ruth Rose - r.s.rose@qmul.ac.uk
The facility has a number of systems suitable for agarose gel electrophoresis, horizontal gel electrophoresis (agarose gel separation of proteins) and vertical gel electrophoresis (SDS-PAGE and native-PAGE). The facility also has western blotting equipment allowing for 3 minute transfer using the TransBlot Turbo (Bio-Rad) as well as the more traditional wet transfer. Please contact Dr Ruth Rose - r.s.rose@qmul.ac.uk
Imaging of gels is performed using a Bio-Rad ChemiDoc™ Imaging System (for chemiluminescence, Coomassie, DNA gel) or a Li-Cor Odyssey (IR-antibodies).