Research
Antibody

Specificity: Human; Applications: FACS IHC IF IP WB

Description and applications

1-  Mouse antibody against Human Melanoma associated Chondroitin Sulphate Proteoglycan (MCSP or NG2) (Subclass: IgG1 Kappa)

The LHM2 antibody is a very good marker for melanoma (>90% stained). LHM2 also stains basal cell carcinomas and subpopulation of basal keratinocytes. LHM2 can be used as a diagnostic panel for immunopathology of malignant melanoma and potential candidates for immunoimaging and immunotherapy of disseminated melanoma. The originators of this antibody report that the MCSP is a cell surface marker specific for epidermal stem cells (J. Invest. Dermatol. in press). Potential applications of the LHM2 antibody include use for separation/isolation of epidermal stem cells, detection of stem cells in skin biopsies or epithelial cell cultures and optimisation of culture conditions to retain epidermal stem cell population in in vitro cultures.

Applications:  FACS IHC IF IP WB

Commercially available at Research and Diagnostics Systems and Novus Biologicals. In collaboration with Nick Tidman

Publications

Somaiah et al. 2012. Clin Cancer Res. 18(19):5479-88. PMID: 22855580.

Ghali et al. 2004. J Invest Dermatol. 122(2):433-42. PMID: 15009727.
 
Kupsch et al. 1999. Clin Cancer Res. 5(4):925-31. PMID: 10213230.
 
Kupsch et al. 1995. Melanoma Res. 5(6):403-11. PMID: 8589614.

Keywords

Antibody, Human Melanoma associated Chondroitin Sulphate Proteoglycan,MCSP, NG2, epidermal stem cells, melanoma, keratinocyte

Prof Irene Leigh

Previously at Blizard Institute

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