DX3 melanoma cell line stably transfected with the integrin beta 6. As described in Hausner et al. Cancer Research (2007) Vol 67, p7833-7840. The human melanoma cell line DX3 was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturer’s instructions (Dynal; Invitrogen).
Useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent DX3 line. DX3 cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of αvβ6 binding peptides.
Hausner et al. 2009. Cancer Res. 69(14):5843-50. PMID: 19549907.
Hausner et al. 2009. Mol Imaging. 8(2):111-21. PMID: 19397856.
Hausner et al. 2007. Cancer Res. 67(16):7833-40. PMID: 17699789.
Barts Cancer Institute
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