Research
Cell line

DX3 melanoma cell line stably transfected with the integrin beta 6

Description and applications

DX3 melanoma cell line stably transfected with the integrin beta 6. As described in Hausner et al. Cancer Research (2007) Vol 67, p7833-7840. The human melanoma cell line DX3 was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturer’s instructions (Dynal; Invitrogen).

Useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent DX3 line. DX3 cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of αvβ6 binding peptides.

Publications

Hausner et al. 2009. Cancer Res. 69(14):5843-50. PMID: 19549907.

Hausner et al. 2009. Mol Imaging. 8(2):111-21. PMID: 19397856.

Hausner et al. 2007. Cancer Res. 67(16):7833-40. PMID: 17699789.

Keywords

Intergrins, cancer, melanoma, αvβ6

Prof John Marshall

Barts Cancer Institute

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