Fractional S-phase sorting using bromodeoxyuridine uptake to study gene replication timing
The determination of the percentage of cells in S-phase is dependent upon the detection of a thymidine analogue, bromodeoxyuridine (BrdU), which when added to culture medium is incorporated into DNA during DNA replication is a well known technique in flow cytometry.
Gene replication timing studies uses the BrdU labelling technique, S phase cells are sorted into 3 or more fractions. Collected fractions are analysed by PCR for genetic analysis after immunoprecipitation of DNA via incorporated BrdU.
- S phase sorted fractions [new window]
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