Blizard Institute

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Specification Comments
Fluorescence sensitivity <200 molecules of equivalent soluble fluoroscein
Fluorescence resolution < 3% for PI labelled chick erythrocyte nuclei
Forward and side scatter sensitivity Sensitivity enables the separation of fixed platelets from noise
Forward and side scatter resolution >10,000 events/sec
Excitation optics
Optical platform Fixed optical assembly, kinematic beam steering
Lasers 20mW Coherent Sapphire 488nm, air cooled, argon ion laser: 530/30nm; 585/42nm; 670nmLP; 780/60nm.
25 mW 405 Coherent VioFlame; 450/50nm; 510/50nm
20 mW 635 JDS Uniphase HeNe; 660/20nm; 780/60nm.
Emission optics
Optical Coupling Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency
Fluorescence Detectors Three fixed fibre apertures
Eight fluorescence detectors High performance, high dynamic range photomultipliers with filters
Signal processing
Workstation resolution Five decades for peak detection, offset less than 1/10,000 full scale
Data acquisition channels Ten acquisition channels with up to three time-displaced laser beams
Dynamic range 18-bit data acquisition with IEEE 32 bit floating-point resolution
262,144 linear channels displayed in a 5-decade logarithmic display
Pulse processing Width and area measurements available for all fluorescence parameters

Ratio measurements for intra-laser parameters
Time Time available correlated to any parameter
Channel triggering Available on any laser signal
Data management
Central processing Dual-Core Intel Xeon processors
Data storage 2GB RAM and 120-GB internal hard drive, internal drive with 250-MG removable disk storage space capacity, 3.5-inch floppy drive, CD-RW drive.
Monitor 24-inch LCD monitor
Software BD FACSDiva v6.1.3, BD FACSCanto clinical v6.1.03
Data File Structure

Flow Cytometry Standard 3.0 or 2.0

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