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Blizard Institute

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Reagent Shop

The Flow Cytometry Core Facility at The Blizard Institute has a free reagent shop from which small supplies of reagents can be used by The Blizard Institute staff before buying these relatively expensive reagents. See below for list of items available and there fluorescent properties and uses.

DNA & RNA Binding Reagents

Probe - click here for details

DAPI 4',6-Diamidino-2-phenylindole dihydrochloride
PI - propidium iodide
7-AAD - 7 actinomycin D
SYTO16
SYTOX ADDvanced
DRAQ7
Hoechst 33342
CyTrack Orange
Pyronin Y

Apoptosis Reagents

Probe - click here for details

Annexin V-FITC
Annexin V-APC
Violet Ratiometric Membrane Asymmetry probe
SYTO16

Calcium Indicators

Probe - click here for details

Indo-1
Rhod-2

Membrane Probes

Probe - click here for details

Bis-oxonol
MitoTracker Orange
MitoTracker Green
Carbocyanine dye, DiIC1(5)
LysoTracker Green
LysoTracker Red
LysoTracker Deep Red
Endoplasmic Reticulum Tracker Green
Endoplasmic Reticulum Tracker Red

Protein Aggregates

Proteosome Reagent

Reactive Oxygen Species Probes

Probe - click here for details

Dihydroethidium

Cell Proliferation Probes

Probe - click here for details

CFSE - 5(6)-Carboxyfluorescein diacetate N-succinimidyl easter
Violet Cell Trace

Antibodies

Anti-LC3B polyclonal (Life Tecnologies)
Anti-LC3B monoclonal (Cell Signalling Technologies)
anti-human CD3-FITC (BD)
anti-human CD3-BB515 (BD)

Secondary Reagents

Goat anti Rabbit-AF-647
Goat anti Rabbit-AF-546
Goat anti Rabbit-AF-405
Goat anti Rabbit-AF-555

General Reagents

Staurosporine
Etoposide
Thapsigargin
Ionomycin
PMA Phorbol 12-myristate 13-acetate
BrdU
RNAse
DMSO
Chloroquine diphosphate
Ethanol
Sodium Azide
Triton X 100
Necrostatin
Flow cytometric Compensation Beads
Flow cytometric absolute count beads

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