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BD LSRII analyser

Specification Comments
Performance
Fluorescence sensitivity <200 molecules of equivalent soluble fluoroscein
Fluorescence resolution < 3% for PI labelled chick erythrocyte nuclei
Forward and side scatter sensitivity Sensitivity enables the separation of fixed platelets from noise
Forward and side scatter resolution >10,000 events/sec
Excitation optics
Optical platform Fixed optical assembly, kinematic beam steering
Lasers 20mW Coherent Sapphire Blue 488nm, 530/30nm; 575/26nm; 610/20nm; 675/20nm; 695/40nm; 780/60nm.
25 mW 405 Coherent VioFlame 440/40nm; 525/50nm; 610/20nm.
20 mW 635 JDS Uniphase HeNe; 660/20nm; 780/60nm or 695/40nm.
355 nm Lightwave Solid-StateUV laser; 450/40nm; 530/30nm.
Emission optics
Optical Coupling Quartz cuvette is coupled to emission lens by refractive index-matching optical gel for optimum collection efficiency
Fluorescence Detectors Four fixed fibre apertures
Thirteen fluorescence detectors High performance, high dynamic range photomultipliers with filters
Signal processing
Workstation resolution Five decades for peak detection, offset less than 1/10,000 full scale
Data acquisition channels Fifteen acquisition channels with up to four time-displaced laser beams
Dynamic range 18-bit data acquisition
262,144 linear channels displayed in a 5-decade logarithmic display
Pulse processing Width and area measurements available for all fluorescence parameters

Ratio measurements for intra-laser parameters
Time Time available correlated to any parameter
Channel triggering Available on any laser signal
Data management
Central processing Dual Intel Core 2 CPU
Data storage 2GB RAM, 300-GB internal hard drive, 3.5-inch floppy drive, CD-RW drive
Monitor Two 18-inch LCD monitors
Software FACSDiva v6.1.03
Data File Structure Flow Cytometry Standard 3.0 or 2.0
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