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Blizard Institute

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Instruments

There are currently five flow cytometers and a LED 4 colour epi-fluorescence microscope in the core facility.

The BD LSR II is an analyser with four lasers, an argon laser (6 colour's), Red HeNe (2 colours), UV laser (2 colours) and violet diode (3 colours) and thus capable of detecting 13 fluorescent signals e.g. FITC, PE, PE-Texas Red or Quantum Dot 605, PE-Cy5, PE-Cy5.5, PE-Cy7, APC, APC-Cy7 or Alexa700, Pacific Blue or BV421, BV511 or Pacific Orange, Quantum Dot 605 and violet and green Indo-1 signals or BUV395 and BUV737. Fluorochrome list and Chromocyte pdf

The BD FACS Canto II is an analyser with three lasers,an argon laser (4 colours), Red HeNe (2 colours) and a violet laser (2 colours) and thus capable of detecting 8 fluorescent signals e.g. FITC, PE, PE-Cy5.5, PE-Cy7, APC, APC-Cy7, Pacific Blue or BV421 and AmCyan or Pacific Orange or BV511. This instrument also has FACS Canto clinical software v2.1 suitable for clinical samples. Fluorochrome list and Chromocyte pdf

The Merck Millipore Muse is a small digital instrument fitted with an Green HeNe with 2 colour PMT detectors. These include PE/PI and PE-Cy5/7-AAD detectors. The 32 bit PMT technology removes the requirement to set voltages on the PMT by the operator in advance of acquiring the samples. The instrument can be used with range of purpose made kits, including annexin V, PI cell cycle and absolute number determinations without the need for the operator to set the compensation. There is a wide range of specially designed kits covering a wide range of biological processes, see link.

The Becton Dickinson FACScan is an analyser with an argon laser capable of detecting 3 fluorescent signals e.g. FITC or GFP, PE and PE-Cy5 or PE-Cy7. Fluorochrome list and Chromocyte pdf

The BD FACSAria IIIu is a cell sorter with four lasers, an blue laser (2 colours), Red HeNe, (3 colours) yellow-green laser (4 colours) and violet diode (6 colours) and currently capable of detecting 15 fluorescent signals. Blue laser excites FITC, PerCP or PercP-Cy5.5: Yellow laser excites PE, PE-Texas Red or PE-CF95, PE-Cy5 orPE-Cy5.5, PE-Cy7: Red laser APC, Alexa700 or APC-Cy7: Violet laser BV421 or Pacific Blue, BV511 or Pacific Orange, BV605, BV650, BV711 and BV780. Chromocyte pdf

The FACSAria can perform 2 and 4 way sorting as well as automatic cell deposition for sorting onto TC plates or slides. Sort rates are 20,000 cells per second for lymphocytes, 15,000 or 10,000 cells per second for cells >10um via 70, 85um and 100um nozzle sizes at 70, 40 and 20 psi respectively.Fluorochrome list.

The Flow Cytometry Core Facility LED fluorescent microscope allows excitation at 4 wavelengths at 355, 425, 470 and 540 nm and emissions to be collected at 420, 450, 530 and 600nm, thus allowing the detection of DAPI, FITC & TRITC. This upright microscope is fitted with 4x, 10x, 40x and 100x objectives. The LED microscope is fitted with a digital 3 MP colour camera connected to a HP computer via a USB 2.0 cable. Images are collected via GXCAPTURE software (GT Vision).

Instruments - Blizard Institute

BD FACSAria IIIu sorter

BD FACS Canto II
Becton Dickinson FACScan Analyser
BD LSR II anaylser
LED Microscope
Merck Millipore Muse Digital Analyser
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