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Flow Cytometry

Flow cytometry is a powerful tool for the multiparameter analysis of cells of all types. Three high powered high speed flow cytometers are available as well as two simpler cytometers and a three colour LED upright Fluorescence microscope in the Blizard Institute. See the Chromocyte list of fluorophores that can be analysed by the Blizard Institute instruments, Aria IIIu, BD LSRII, BD FACSCanto II, BD FACScan.

Download a Flow Cytometry Core Facility Welcome Pack for a brief overview of the Core Facility, see pdf.

SPADE software (fluidigm inc), a year subsrciption was purchased by Themes funding for Users to allow multi-dimensional analysis of FACS data by Spanning Tree Progression of Density Normalized Events or SPADE, see the link https://fluidigm.cytobank.org/cytobank/ please enquire for login details, see the link for the capabilities of the software, pdf. The software allows the automated identification of subpopulations of cell samples labelled with up to 40 fluorophores.

New training, assistance and sorting reporting forms for giving feedback to the Flow Cytometry Core Facility. For out of hours work download help guides for the Canto II & LSRII.

NEW MIFlowCyte criteria for good practice in data publication, see Cytometry the scientific journal for the International Society for the Advancement of Cytometry, ISAC, see details and download form in Flow Cytometry Guides. All such data for publication in Cytometry has to be uploaded to FlowRepository.org

Sorter COSHH Form

All cell samples for sorting must be assessed for pathogenic and GM potential. Thus it is also required to supply a BioCOSHH form assessment of the cells due for sorting. Fixed samples can be sorted after the fixative is removed. More information can be found on the link, biological hazard. Cell sorting assessment form (downloadable pdf), Cell Sorting Biosafety Guidance, and QMUL Biological Safety Procedures documentation.

NEW ASSAY Design & Expert Assistance!

A REMINDER to all staff that the Flow Cytometry Core Facility offers NEW ASSAY DEVELOPMENT expertise, click here for list of new assay's already developed. Flow cytometry may be the answer to a research project problem and we can investigate whether flow cytometry can be used to investigate your research demands.

Expert support and technical assistance is also available to help with the design and development of experiments. Please see contacts for help and advice. Academic Lead for this core facility is Dr Dan Pennington,The Immune Systems Lead.

NEW BOOKING SYSTEM FOR 2014 Blizard Institute use only:- Click here for on-line booking https://www.quartzy.com/login

ICMS Staff and associated Institutes

£30 per hour for LSR II or Canto II analysis

£10 per hour for the Millipore Muse

£5 per hour for FACScan analysis

£50 per hour for assistance

£50 per hour for sorting

External Academic

£50 per hour for analysis

£85 per hour for assistance

£100 per hour for sorting

Industrial

£70 per hour for analysis

£120 per hour for assistance

£175 per hour for sorting

  • SPADE software (fluidigm inc), a year subsrciption (September 2014) was purchased by Themes funding for Users to allow multi-dimensional analysis of FACS data by Spanning Tree Progression of Density Normalized Events or SPADE, see the link https://fluidigm.cytobank.org/cytobank/ please enquire for login details, see the link for the capabilities of the software, pdf [PDF 1KB]
  • New HP laser Jet printer for Flowjo printing purchased by Themes funds
  • New iMAC with FlowJo version 10 - from Themes funding
  • Aria I upgrade in Feb 2014 to Aria IIIu - new Yellow-Green 561nm laser with 15 colour detection - purchased by User Funds

Dr Gary Warnes, Flow Cytometry Core Facility Manager
Tel: 020 7882 2402
email: g.warnes@qmul.ac.uk

Prof Dan Pennington, Immune Systems Research Lead, Centre for Immunology & Infectious Diseases
Tel: 020 7882 2302
email: d.pennington@qmul.ac.uk

Blizard Users for on-line booking please go to https://www.quartzy.com/facilities/calendar

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